Antifungal substances and process for their production

ABSTRACT

There is disclosed a series of macrolides isolated from the fermentation broth of a microorganism identified as MA-5000 which morphological analysis reveals to be a strain of Streptomyces hygroscopicus. The compounds structures are presented based upon analytical studies. The compounds have antifungal activity.

This is a division of application Ser. No. 07/202,612, filed June 6, 1988, U.S. Pat. No. 5,028,537, which is a continuation-in-part of application Ser. No. 791,623, filed Oct. 25, 1985, abandoned which is a division of application Ser. No. 593,448, filed Mar. 26, 1984. U.S. Pat. No. 4,575,500

SUMMARY OF THE INVENTION

This invention is concerned with novel organic chemical compounds. In particular, it is concerned with phosphate esters which are produced by the fermentation of a nutrient medium with a strain of the microorganism Streptomyces hygroscopicus MA-5000. Thus, it is an object of this invention to provide for such novel compounds, and a method for preparing such products microbiologically. It is a further object of this invention to provide for the recovery and purification of such compounds from the fermentation broth. These substances have antifungal activity, and it is, thus, an additional object of this invention to provide for novel antifungal compositions containing the disclosed compounds. Further objects of this invention will become apparent from the following description of this invention.

DESCRIPTION OF THE INVENTION

In accordance with this invention, there is described a series of novel substances which are prepared by growing under controlled conditions, a previously undescribed strain of a microorganism. The novel compounds are produced by Streptomyces hygroscopicus MA-5000. The compounds are obtained by fermentation and recovered in substantially pure form as described herein.

Based on taxonomic studies, the microorganism capable of producing these compounds is of a new strain of the microorganism Streptomyces hygroscopicus. One such culture, isolated from soil, is designated MA-5000 in the culture collection of Merck & Co., Inc., Rahway, N.J. A sample of this culture, capable of producing the herein described compound, has been deposited, without restriction as to availability, in the permanent culture collection of the American Type Culture Collection at 12301 Parklawn Drive, Rockville, Md. 20852, and has been assigned the accession number ATCC 39476.

The morphological and cultural characteristics of Streptomyces hygroscopicus MA-5000 are set forth below:

CULTURAL CHARACTERISTIC OF

Streptomyces hygroscopicus MA-5000 - ATCC 39476 (V-vegetative growth; A=aerial mycelium; SP=soluble pigment)

Morphology: Sporophores form compact spirals along aerial hyphae. As culture ages, spore chains coalesce to form dark moist areas. Spore surface by electron microscope (TEM) shows a smooth surface on some spores and a rough to warty surface on others.

Oatmeal agar (ISP Medium 3)

V: Reverse - brown edged with dark brown

A: Dark gray mixed with white and light gray, becoming black and moist with age.

SP: Lt. grayish-brown.

Czapek Dox agar (sucrose nitrate agar)

V: Reverse - dark brown

A: moderate, grayish white

SP: Lt. brown

Egg albumin agar

V: Flat, spreading, grayish-brown

A: Light gray mixed with white and edged with dark gray, some areas becoming moist and black.

SP: Light brown

Glycerol asparagine agar (ISP Medium 5)

V: Reverse - dark yellow brown

A: Light gray mixed with white and yellowish white. Droplets of golden yellow exudate are present.

SP: Lt. tan

Inorganic salts-starch agar (ISP Medium 4)

V: Reverse - light gray-brown edged with dark gray

A: Dark gray mixed with light gray and white, becoming black and moist.

SP: Light grayish-brown

Yeast extract-malt extract agar (ISP Medium 2)

V: Reverse - dark yellow brown

A: Dark gray becoming black and moist with age. Droplets of golden yellow exudate also present.

SP: Light brown

Peptone-iron-yeast extract agar

V: tan

A: sparse, whitish

SP: None

Melanin: negative

Nutrient tyrosin agar

V: Rev. - brown

A: Moderate, light gray

SP: Light brown

Tyrosine Agar (ISP Medium 7)

V: Reverse - dark brown

A: Gray edged with dark gray; droplets of golden yellow exudate present

SP: Light brown

Carbon utilization

Pridham-Gottlieb basal medium+1% carbon source;

+=growth; ±=growth poor or questionable;

-=no growth as compared to negative control (no carbon source)

    ______________________________________                                                Glucose +                                                                      Arabinose                                                                              ±                                                                   Cellulose                                                                              --                                                                     Fructose                                                                               +                                                                      Inositol                                                                               +                                                                      Lactose ±                                                                   Maltose +                                                                      Mannitol                                                                               +                                                                      Mannose +                                                                      Raffinose                                                                              ±                                                                   Rhamnose                                                                               ±                                                                   Sucrose +                                                                      Xylose  ±                                                            ______________________________________                                    

Temperature range (Yeast extract-dextrose+salts agar)

26° C. - Good vegetative growth and sporulation

37° C. - Good vegetative growth scant aerial mycelia

50° C. - No growth

Oxygen requirement (Stab culture in yeast extract-dextrose+salts agar)

Aerobic

All readings taken after three weeks at 28° C. unless noted otherwise. pH of all media approximately neutral (6.8-7.2)

A careful comparison of the foregoing data with published descriptions, including Bergey's Manual of Determinative Bacteriology 8th ed (1974); Waksman, The Actinomycetes Vol. II (1961); International Journal of Systematic Bacteriology 18, 68-189, 279-392 (1968); 19, 391-512 (1969); 22, 265-394 (1972); shows a close correlation between the description of a bacterium identified as Streptomyces hygroscopicus and he morphological and cultural characteristics of MA-5000. It is therefore determined that MA-5000 is a new strain of the known species Streptomyces hygroscopicus.

The above description is illustrative of a strain of Streptomyces hygroscopicus MA-5000 which can be employed in the production of the instant compound. However, the present invention also embraces mutants of the above described microorganism. For example, those mutants which are obtained by natural selection or those produced by mutating agents including ionizing radiation such as ultraviolet irradiation, or chemical mutagens such as nitrosoguanidine or the like treatments are also included within the ambit of this invention.

The instant compounds are produced during the aerobic fermentation of suitable aqueous nutrient media under conditions described hereinafter, with a producing strain of Streptomyces hygroscopicus MA-5000. Aqueous media such as those used for the production of many antibiotic substances are suitable for use in this process for the production of this macrocyclic compound.

Such nutrient media contain sources of carbon and nitrogen assimilable by the microorganism and generally low levels of inorganic salts. In addition, the fermentation media may contain traces of metals necessary for the growth of the microorganisms, and production of the desired compound. These are usually present in sufficient concentrations in the complex sources of carbon and nitrogen, which may be used as nutrient sources, but can, of course, be added separately to the medium if desired.

In general, carbohydrates such as sugars, for example dextrose, sucrose, maltose, lactose, dextran, cerelose, corn meal, oat flour, and the like, and starches are suitable sources of assimilable carbon in the nutrient media. The exact quantity of the carbon source which is utilized in the medium will depend, in part, upon the other ingredients in the medium, but it is usually found that an amount of carbohydrate between 0.5 and 5% by weight of the medium is satisfactory. These carbon sources can be used individually or several such carbon sources may be combined in the same medium.

Various nitrogen sources such as yeast hydrolysates, yeast autolysates, yeast cells, tomato paste, corn meal, oat flour, soybean meal, caesin hydrolysates, yeast extracts, corn steep liquors, distillers solubles, cottonseed meal, meat extract and the like, are readily assimilable by Streptomyces hygroscopicus MA-5000 in the production of the instant compound. The various sources of nitrogen can be used alone or in combination in amounts ranging from 0.2 to 6% by weight of the medium.

Among the nutrient inorganic salts, which can be incorporated in the culture media are the customary salts capable of yielding sodium, potassium, magnesium, ammonium, calcium, phosphate, sulfate, chloride, carbonate, and like ions. Also included are trace metals such as cobalt, manganese, and the like. For the best production of the instant compound, the addition of calcium carbonate to the production medium is most preferred.

It should be noted that the media described hereinbelow and in the Examples are merely illustrative of the wide variety of media, which may be employed, and are not intended to be limitative.

The following are Examples of media suitable for growing strains of Streptomyces hygroscopicus MA-5000.

    ______________________________________                                         Medium A                                                                       Dextrose                 1.0     g.                                            Soluble starch           10.0    g.                                            Beef extract             3.0     g.                                            Yeast autolysate (As ardamine                                                                           5.0     g.                                            PH available from Yeast                                                        Products Inc., Clifton, N.J.)                                                  NZ Amine-E (caesin hydrolysate-                                                                         5.0     g.                                            available from Humko-Sheffield                                                 Memphis, Tenn.)                                                                MgSO.sub.4.7H.sub.2 O    0.05    g.                                            Phosphate Buffer         2.0     ml                                            CaCO.sub.3               0.5     g.                                            Distilled water          1000    ml.                                           pH                       7.0-7.2                                               Phosphate Buffer                                                               KH.sub.2 PO.sub.4        91.0    g                                             Na.sub.2 HPO.sub.4       95.0    g                                             Distilled water          1000    ml                                            pH                       7.0                                                   Medium B                                                                       Tomato paste             20.0    g.                                            Primary Yeast            10.0    g.                                            Dextrin (CPC starch)     20.0    g.                                            CoCl.sub.2.6H.sub.2 O    0.005   g.                                            Distilled water          1000    ml.                                           pH                       7.2-7.4                                               Medium C                                                                       Corn meal                20.0    g.                                            Distillers solubles      10.0    g.                                            Soybean meal             15.0    g.                                            Sodium citrate           4.0     g.                                            CaCl.sub.2.2H.sub.2 O    0.5     g.                                            MgSO.sub.4.7H.sub.2 O    0.1     g.                                            CoCl.sub.2.6H.sub.2 O    0.01    g.                                            FeSO.sub.4.2H.sub.2 O    0.01    g.                                            Polyglycol P2000 (Polypropylene glycol                                                                  2.5     mg.                                           mw 2000)                                                                       Distilled water          1000    ml.                                           pH                       6.5                                                   Medium D                                                                       Lactose                  20.0    g.                                            Distillers solubles      15.0    g.                                            Autolyzed yeast (Ardamine PH)                                                                           5.0     g.                                            Distilled water q.s. to  1000    ml.                                           pH                       7.0                                                   Medium E                                                                       Tomato paste             40.0    g.                                            Oat flour                10.0    g                                             Distilled water          1000    ml.                                           pH                       7.0                                                   Medium F                                                                       Corn Steep Liquor        15.0    g.                                            (NH.sub.4).sub.2 SO.sub.4                                                                               4.0     g.                                            CaCO.sub.3               6.0     g                                             Soluble Starch           20.0    g.                                            Corn meal                1.0     g.                                            Soybean meal             4.0     g.                                            Glucose                  5.0     g.                                            KH.sub.2 PO.sub.4        0.3     g                                             Lard oil                 2.5     g.                                            Distilled water          1000    ml.                                           pH                       6.7                                                   Medium G                                                                       Dextrose                 10.0    g                                             Asparagine               1.0     g                                             K.sub.2 HPO.sub.4        0.1     g                                             MgSO.sub.4.7H.sub.2 O    0.5     g                                             Yeast Extract            0.5     g                                             Oat Flour                10.0    g                                             CaCO.sub.3               3.0     g                                             Trace Element Mix        10.0    ml                                            Distilled water          1000    ml                                            Adjust pH to             7.2                                                   Trace Element Mix                                                              FeSO.sub.4.7H.sub.2 O    1000    mg                                            MnSO.sub.4.4H.sub.2 O    1000    mg                                            CuCl.sub.2.2H.sub.2 O    25      mg                                            CaCl.sub.2.2H.sub.2 O    100     mg                                            H.sub.3 BO.sub.3         56      mg                                            (NH.sub.4).sub.6 MO.sub.4 O.sub.24.6H.sub.2 O                                                           19      mg                                            ZnSO.sub.4.7H.sub.2 O    200     mg                                            Distilled water          1000    ml                                            Medium H                                                                       Medium G                 1000    ml                                            Oat Flour                10      g                                             pH                       7.2                                                   ______________________________________                                    

The fermentation employing Streptomyces hygroscopicus MA-5000 can be conducted at temperatures ranging from about 20° C. to about 40° C. For optimum results, it is most convenient to conduct these fermentations at a temperature in the range of from about 24° C. to about 30° C. Temperatures of about 27°-28° C. are most preferred. The pH of the nutrient medium suitable for producing the instant compounds can vary from about 5.0 to 8.5 with a preferred range of from about 6.0 to 7.5.

Small scale fermentations are conveniently carried out by placing suitable quantities of nutrient medium in a flask employing known sterile techniques, inoculating the flask with either spores or vegetative cellular growth of Streptomyces hygroscopicus MA-5000 loosely stoppering the flask with cotton and permitting the fermentation to proceed in a constant room temperature of about 28° C. on a rotary shaker at from 95 to 300 rpm for about 2 to 10 days. For larger scale work, it is preferable to conduct the fermentation in suitable tanks provided with an agitator and a means of aerating the fermentation medium. The nutrient medium is made up in the tank and after sterilization is inoculated with a source of vegetative cellular growth of Streptomyces hygroscopicus MA-5000. The fermentation is allowed to continue for from 1 to 8 days while agitating and/or aerating the nutrient medium at a temperature in the range of from about 24° to 37° C. The degree of aeration is dependent upon several factors such as the size of the fermenter, agitation speed, and the like. Generally the larger scale fermentations are agitated at about 95 to 300 RPM and about 2 to 20 cubic feet per minute (CFM) of air.

The novel compound of this invention is found primarily in the mycelium on termination of the Streptomyces hygroscopicus MA-5000 fermentation and may be removed and separated therefrom as described below.

The separation of the novel compound from the whole fermentation broth and the recovery of said compounds is carried out by solvent extraction and application of chromatographic fractionations with various chromatographic techniques and solvent systems.

The instant compound has slight solubility in water, but is soluble in organic solvents. This property may be conveniently employed to recover the compound from the fermentation broth. Thus, in one recovery method, the whole fermentation broth is combined with approximately an equal volume of an organic solvent. While any organic solvent may be employed, it is preferable to use a water-immiscible solvent such as ethyl acetate, methylene chloride, chloroform and the like. Generally several extractions are desirable to achieve maximum recovery. The solvent removes the instant compound as well as other substances lacking the antifungal activity of the instant compound. If the solvent is a water immiscible one, the layers are separated and the organic solvent is evaporated under reduced pressure. The residue is placed onto a chromatography column containing preferably, silica gel. The column retains the desired products and some impurities, but lets many of the impurities, particularly the non-polar impurities, pass through. The column is washed with a moderately polar organic solvent such as methylene chloride or chloroform to further remove impurities, and is then washed with a mixture of methylene chloride or chloroform and an organic solvent of which acetate, methanol, and ethanol and the like are preferred. The solvent is evaporated and the residue further chromatographed using column chromatography, thin layer chromatography, preparative layer chromatography, high pressure liquid chromatography and the like, with silica gel, aluminum oxide, ion exchange resins, dextran gels and the like, as the chromatographic medium, with various solvents and combinations of solvents as the eluent. Thin layer, high pressure, liquid and preparative layer chromatography may be employed to detect the presence of, and to isolate the instant compound. The use of the foregoing techniques as well as others known to those skilled in the art, will afford purified compositions containing the instant compound. The presence of the desired compounds is determined by analyzing the various chromatographic fractions for physico-chemical characteristics. The structures of the instant compounds have been determined by detailed analysis of the various spectral characteristics of the compounds, in particular their nuclear magnetic resonance, mass, ultraviolet and infrared spectra.

The mass spectral data were obtained on the trimethylsilyl derivatives of the instant compounds, prepared by treatment with bis(trimethylsilyl)-trifluoroacetamide (BSTFA), 10:1 (v/v) in pyridine under nitrogen at room temperature for 18 hours to afford molecular ions in their electron impact mass spectra. Analogous silylation with perdeutero-BSTFA allowed unambiguous determination of the number of silyl groups in each molecule. High resolution exact mass measurements indicated the following molecular formulae. (The underivatized nominal molecular weights and molecular formulae have been included in the table for clarity.)

    __________________________________________________________________________     Com-                                                                               Neat                                                                               Molecular                                                                             Corresponding                                                                          Silyl                                                                               Silyl                                              pound                                                                              Weight                                                                             Formula                                                                               Calculated                                                                             Found                                                                               Derivative                                         __________________________________________________________________________     I   599 C.sub.29 H.sub.46 NO.sub.10 P                                                         959.4836                                                                               959.4847                                                                            C.sub.29 H.sub.46 NO.sub.10 P + (C.sub.3                                       H.sub.8 Si).sub.5                                  II  513 C.sub.25 H.sub.40 NO.sub.8 P                                                          873.4468                                                                               873.4452                                                                            C.sub.25 H.sub.40 NO.sub.8 P + (C.sub.3                                        H.sub.8 Si).sub.5                                  III 613 C.sub.30 H.sub.48 NO.sub.10 P                                                         973.4992                                                                               973.4993                                                                            C.sub.30 H.sub.48 NO.sub.10 P + (C.sub.3                                       H.sub.8 Si).sub.5                                  IV  614 C.sub.30 H.sub.47 O.sub.11 P                                                          974.4832                                                                               974.4766                                                                            C.sub.30 H.sub.47 O.sub.11 P + (C.sub.3                                        H.sub.8 Si).sub.5                                  __________________________________________________________________________

The nuclear magnetic resonance (NMR) spectrum for each of Compounds I, II, III and IV are shown in attached FIGS. 1 to 4 respectively. The spectra for Compounds I, III and IV were recorded in CD₃ OD at 60° C. and that for Compound II in CD₃ OD at 25° C. A small amount of ND₄ OD was added to Compound II to effect complete dissolution. Chemical shifts are shown in ppm relative to internal tetramethylsilane (TMS) at 0 ppm.

Based on these experimental data, the instant compounds are believed to have the following structural formula: ##STR1## wherein the compounds have the following values for R₁, R₂ and R₃ :

    ______________________________________                                         R.sub.1            R.sub.2   R.sub.3                                           ______________________________________                                                 ##STR2##       NH.sub.3.sup.⊕                                                                       OPO.sub.3 H.sup.⊖                     II     H               NH.sub.3.sup.⊕                                                                       OPO.sub.3 H.sup.⊖                     III                                                                                    ##STR3##       NH.sub.3.sup.⊕                                                                       OPO.sub.3 H.sup.⊖                     IV                                                                                     ##STR4##       OH        OPO.sub.3 H.sub.2                             ______________________________________                                    

Fields of technology adversely affected by the lack of effective fungicides are many and include the paint, wood, textile, cosmetic, leather, tobacco, fur, rope, paper, pulp, plastics, fuel, rubber and food industries. Fungicides are also utilized for agricultural application, for instance in preventing or minimizing fungus growth on plants, fruits, seeds or soil.

Although many antifungal agents have been described and used heretofore in an effort to control fungi, none are entirely satisfactory and continued losses resulting from fungal attack make the problem of control a serious and lasting one. The number of fungicides practically useful in combatting fungal growth have been small and only in a few cases have synthetic organic chemicals been found applicable.

The compounds of the invention are effective in controlling the growth of Aspergillus species, for example A. niger, A. flavus, A. fumigatus, A. oryzae, A. luchensis, A. versicolor, A. sydowi, A. nidulans, A. flaucus and A. terreus, Penicillium species, for example, P. notatum, P. roqueforti, P. chrysogenum, P. oxalicum, P. spinulosum, P. martensii, P. citrinium, P. digitatum, P. expansum, P. italicum, P. cyclopium, and P. funiculosum, Neurospora series such as N. sitophila, Phoma species such as P. terrestrius, Rhizopus species, Alternaria species such as A. solani, Chaetomium species such as C. globosum, Chaetomicum species, for example, C. clivaceum, and Monilia species such as M. sitophila and M. nigra. The above fungi may be found on cosmetics, leather, electrical insulation, textiles, and numerous other materials capable of supporting their growth.

The compounds of this invention may be employed in treatment of plants, soils, fruits, seeds, fur, wood and the like. The fungicidal effectiveness of these compounds has been demonstrated against soil fungi, such as Rhizoctonia solani, Fusarium solani, and Pythium ultimum, plant fungi, for instance Erysiphe polygoni and Alternaria solani as well as against saprophytes known to attack wood, pulp and lumber such as Lenzites trabea and Ceratocystis pilifera and the fungus Pullularia pullalans which attacks paint.

In particular the compounds of this invention are useful in controlling those agricultural fungus infections that attack growing plants. The compounds are particularly effective against those fungi, which cause rice blast, tomato late blight, tomato early blight, wheat leaf rust, bean powdery mildew and tomato Fusarium wilt.

It should be understood that the compounds may be utilized in diverse formulations, solid, including finely divided powders and granular materials as well as liquid, such as solutions, emulsions, suspensions, concentrates, emulsifiable concentrate, slurries and the like, depending upon the application intended and the formulation media desired.

Thus it will be appreciated that compounds of this invention may be employed to form fungicidally active compositions containing such compounds as essentially active ingredients thereof, which compositions may also include finely divided dry or liquid diluents, extenders, fillers, conditioners and excipients, including various clays, diatomaceous earth, talc, and the like, or water and various organic liquids such as lower alkanols, for example ethanol and isopropanol, or kerosene, benzene, toluene and other petroleum distillate fractions or mixtures thereof.

When the active agents are employed in preventing topical fungal growth, one or more of the compounds may be uniformly distributed in a vehicle that is chemically compatible with the particular compound selected, non-inhibiting with respect to the action of the antifungal and essentially non-injurious to body tissue under the conditions of use.

It should be understood that the compounds of the invention may be used in combination one with the other as well as with other fungicidally active materials. For instance, a mixture of the active compounds with 2-(4'-thiazolyl)benzimidazole sorbic acid or its salts, propionic acid or its salts, mycostatin, sodium diacetate, trichomycin, amphotericin, griseofulvin, undecylenic acid, chlorquinadol, 5,7-dichloro-8-hydroxyquinoline (Vioform), sodium o-phenylphenate, o-phenylphenol, biphenyl, chlorinated phenols, sodium benzoate, dehydroacetic acid and its salts or esters of parahydroxybenzoic acid, such as the methyl and propyl ester (parabens) can be used to give fungicidal effect when used in appropriate concentrations. It is quite clear, too, that the compounds defined above may be used in conjunction with effective antibacterial materials in appropriate instances so as to combine the action of each in such a situation as to be particularly useful, for instance, in applications where the presence of bacteria creates undesirable results alongside the detrimental action of fungi.

It has been found that growth of various fungi existing in soil is limited or terminated by the addition to the soil of minor quantities of the compounds described. The term soil as used herein is intended to include all media capable of supporting the growth of plants and may include humus, sand, manure, compost, artificially created plant growth solution, and the like.

We have also found that the fungicides of the invention are effective against fungal diseases of plants, and may be effectively used either by direct contact with the foliage or systemically, by introduction through the roots.

The following examples are being provided in order that the instant invention may e more fully understood. Such examples are not to be construed as being limitative of the invention.

EXAMPLE 1 1. "A" Stage

Culture MA 5000 is maintained in the lyophilized state in a 1.0 ml ampoule containing 0.15 ml of a skim milk suspension of the culture.

2. "B" Stage

Vessel: 250 ml 3 baffled Erlenmeyer flask containing 50 ml medium per flask.

    ______________________________________                                                          Wt/Vol                                                        ______________________________________                                         Medium:                                                                        Dextrose           0.1%                                                        Soluble Starch     1.0%                                                        Beef Extract       0.3%                                                        Ardamine PH        0.5%                                                        NZ-Amine Type E    0.5%                                                        MgSO.sub.4.7H.sub.2 O                                                                             0.005%                                                      1.34M Phosphate Buffer                                                                            0.02%      vol/vol                                          CaCO.sub.3 (after pH adjustment)                                                                  0.05%                                                       Phosphate Buffer                                                               KH.sub.2 PO.sub.4  9.1%                                                        Na.sub.2 HPO.sub.4 9.5%                                                        ______________________________________                                    

Inoculum: Contents of one lyophilization tube into each "B" flask

Incubation: 48 hours at 28° C. on a rotary shaker with a 2" throw rotating at 220 RPM.

Sterility: Streak plates and gram stain

3. "C" Stage

Vessel: 2-liter 3 baffled Erlenmeyer flask containing 500 ml medium

Medium: Same as "B" Stage

Inoculum: 10 ml from "B" Stage

Incubation: Same as "B" Stage

Sterility: Same as "B" Stage

4. "D" Stage

Vessel: 189-liter stainless steel fermentor containing 160 liters of medium

    ______________________________________                                         Medium:                                                                        ______________________________________                                         Dextrose (Cerelose)                                                                             160          gms                                              Starch Modified CPC                                                                             1600         gms                                              Meat Extract     480          gms                                              Ardamine pH      800          gms                                              NZ Amine Type E  800          gms                                              MgSO.sub.4.7H.sub.2 O                                                                           8            gms                                              KH.sub.2 PO.sub.4                                                                               29           gms                                              Na.sub.2 HPO.sub.4                                                                              30           gms                                              pH to            7.0-7.2                                                       Add CaCO.sub.3   80           gms                                              ______________________________________                                    

Sterilization: 121° C. for 15 minutes

Inoculum: 500 ml from "C" Stage

Incubation:

a) Temp: 28° C.

b) Airflow 3 ft³ /min

c) Agitation 150 RPM

d) Pressure 30 psig

e) Cycle 48 hrs

Defoamer: Polyglycol 2000

Sterility: Microscopic examination and YED streak plates at 28° C. and 37° C.

5. "E" Stage

Vessel: 756-liter fermentor containing 467 liters of medium

    ______________________________________                                         Medium:       Fermentation 1                                                                             Fermentation 2                                       ______________________________________                                         Corn Meal     9340    gms     9680   gms                                       Distillers Solubles                                                                          4670    gms     4840   gms                                       Soybean Meal  7005    gms     7260   gms                                       CaCl.sub.2.2H.sub.2 O                                                                        233.5   gms     242    gms                                       MgSO.sub.4.7H.sub.2 O                                                                        46.7    gms     48.4   gms                                       CoCl.sub.2.6H.sub.2 O                                                                        4.7     gms     4.8    gms                                       FeSO.sub.4.7H.sub.2 O                                                                        4.7     gms     4.8    gms                                       CaCO.sub.3    1868    gms     1936   gms                                       Polyglycol P-2000                                                                            116     ml      1.2    l                                         pH to         6.5             6.5                                              Volume        467     liters  484    liters                                    ______________________________________                                    

Sterilization: 121° C. for 15 minutes

Inoculum: 43 liters from "D" Stage

Incubation:

a) Temp 28° C.

b) Airflow 10 ft³ /min

c) Agitation 130 RPM

d) Pressure 13 psig

e) Cycle 3 days

Defoamer: Polyglycol P-2000

Sterility: Microscopic examination and YED streak plates at 28° C. and 37° C.

Harvest: Into drums for work-up as in Examples 2 and 3

EXAMPLE 2

Four hundred liters of whole broth from Example 1 "E" Stage fermentation 1, were filtered through a press after the addition of celite, a filter aid. The mycelial cake was extracted with 32 liters of 50% aqueous acetone for one hour. This slurry was filtered and the extract concentrated in vacuo to an aqueous solution. This concentrate was added to the filtered broth and the entire solution was extracted twice with a half volume of methylene chloride. The aqueous phase was then passed through 40 liters Dowex 1X2 (C1⁻) column. After a water wash the column was eluted with 120 liters of 5% sodium chloride followed by 120 liters of 3% ammonium chloride in 90% methanol. The second eluate was concentrated in vacuo to 4 liters and eluted to 16 liters with water. This solution was passed through a 16 liter Amberlite XAD-2 column. After a water wash the column was eluted with 48 liters 10% aqueous acetone, 48 liters 20% aqueous acetone and 50% aqueous acetone. The third eluate was concentrated to an aqueous solution of 4 liters which was extracted twice with 8.8 liters butanol. The butanol extracts were concentrated to a small volume and the activity precipitated with the addition of hexane. The precipitate was chromatographed on a 500 ml Mallinkrodt CC-7 silica gel column in acetone with stepwise increases of methanol in acetone. Active cuts were combined and chromatographed on a 225 ml LH-20 column in methanol. Active cuts were combined and rechromatographed on the same column in 25% acetone/methanol yielding 549 mg. Aliquots of this purified preparation were chromatographed on a preparative RP-2, 10 μm HPLC column (9 mm×30 cm) using a methanol-1% HOAc gradient system. Fractions of the three separated components were combined, concentrated to aqueous solutions and freeze-dried to yield the following samples:

Component I 18 mg

Component II 16 mg

Component III 14 mg

The remainder of the LH-20 rich cut was chromatographed on a preparative RP-18, 10 μm HPLC column (9 mm×30 cm) using the isocratic system 500/450/50 methanol/water/1.0 M PO₄ pH 6.5 to yield the following samples:

Component I 15.8 mg

Component II 9.9 mg

EXAMPLE 2

The fermentation of Example 1 using "E" Stage fermentation 2 was carried out twice and each 400 liter fermentation was filtered 320 liters through a press after addition of celite. Each mycelial cake was extracted with 320 liters of 50% aqueous acetone for one hour. The acetone extracts were filtered and concentrated to aqueous solutions. These extracts and the filtrates were individually extracted with a half volume of methylene chloride and then each extracted twice with a half volume of butanol. The butanol extracts were combined and evaporated onto 8 liters Grace silica gel. The coated gel was eluted sequentially with ethyl acetate, acetone, 10% methanol/acetone and 100% methanol. The methanol eluate was concentrated (180 gm) onto 700 ml Baker silica gel and placed on top of a two-liter silica gel column. This column was eluted with acetone, then stepwise with 20-40-75% methanol/acetone. The active cuts were concentrated, extracted with butanol (16 gm) and chromatographed on a two-liter EM 25-40 μm RP C-18 column with isocratic elution of 60/40 methanol/0.01 M PO₄ pH 6.5. Cuts were combined based on their zone size and analytical HPLC analysis and rechromatographed on a 200 ml EM 25-40 μm RP C-18 column using 55/45 methanol/buffer. Final purification of the individual active components was achieved by chromatography on a 10 μm RP C-18 column, 9 mm×50 cm.

Component II 40 mg

Component III 6.7 mg

Component IV 2.5 mg 

What is claimed is:
 1. A compound produced by fermenting a strain of Streptomyces hygroscopicus MA 5000, ATCC 39476 in aqueous nutrient medium containing an assimilable source of carbon, an assimilable source of nitrogen and inorganic salts, and being one of the following or a mixture thereof:(a) Compound I, a compound having a molecular weight of 599, a molecular formula of C₂₉ H₄₆ NO₁₀ P and a NMR spectrum substantially as shown in FIG. 1 of the accompanying drawings; (b) Compound II, a compound having a molecular weight of 513, a formula of C₂₅ H₄₀ NO₈ P and a NMR spectrum substantially as shown in FIG. 2 of the accompanying drawings; (c) Compound III, a compound having a molecular weight of 613, a molecular formula of C₃₀ H₄₈ NO₁₀ P and a NMR spectrum substantially as shown in FIG. 3 of the accompanying drawings; or (d) Compound IV, a compound having a molecular weight of 614, a molecular formula of C₃₀ H₄₇ O₁₁ P and a NMR spectrum substantially as shown in FIG. 4 of the accompanying drawings.
 2. A compound having the formula: ##STR5## wherein the compounds have the following values for R₁, R₂ and R₃ :

    ______________________________________                                         R.sub.1           R.sub.2   R.sub.3                                            ______________________________________                                               ##STR6##        NH.sub.3.sup.⊕                                                                       OPO.sub.3 H.sup.⊖                      II   H                NH.sub.3.sup.⊕                                                                       OPO.sub.3 H.sup.⊖                      III                                                                                  ##STR7##        NH.sub.3.sup.⊕                                                                       OPO.sub.3 H.sup.⊖                      IV                                                                                   ##STR8##        OH        OPO.sub.3 H.sub.2.                             ______________________________________                                     